Magaji Sanusi
Department of Science Laboratory, Nigeria
Title: Problems associated with small scale plasmid DNA isolation, restriction digestion and agarose gel electrophoresis
Biography
Biography: Magaji Sanusi
Abstract
Two experiments on alkaline lysis method of plasmid DNA isolation was carried out utilizing strains of E.coli JM109, a non- pathogenic bacteria that has been deliberately disabled. Restriction digests and gel electrophoresis carried out. The result of the first gel electrophoresis reveals only traces of RNA, hence no DNA fragments on the samples loaded labeled A to D this could be attributed to possible contamination from the procedures. For the second gel electrophoresis samples, A is plasmid free, B and C contains small fragments approximately 2.3 to 5.6 and D contains the fertility plasmids.